Two people fitting the description for each skeleton have been reported missing in the past year. Valves direct blood flow so it does not flow backwards in the heart. So what we were in charge of doing was to evaluate the bones and to see what the age, height, gender, and ethnic of this person was. Use information from the sites to complete the activity described in Part B of the Student Response Sheet. Then use a scalpel to cut through the entire length of the right atrium and and left ventricle.
You do not want to contaminate your samples. It is also important to take finger prints and collect and examine hair found at the scene as well. Allow the samples to cool a few minutes before loading them on the gel. Prior to the test patients must fast for 12 hours, and at the beginning of the test their blood is drawn as a control for their baseline blood glucose level and they must drink a concentrated sugar solution. . Make sure your explanation is clear and well supported by what you see in the gel and provide a clear conclusion as to the identity of the skeleton in the park. What are restriction fragment length polymorphisms? To begin, we followed out an experiment with the enzyme catalase and hydrogen peroxide H2O2.
Tissue is made up of many cells, the building blocks of life. Blood is then drawn at different time intervals to monitor if the glucose is being broken down in the body. To the left is a graph that shows all four of our trials. All other reagents should be located at your lab station. It is also important to be able to understand the trends of the graphs of glucose concentration of blood in order to diagnose if a patient has Type 1 or Type 2 diabetes.
Secondly, we created our own experiment. Each tube will have a final volume of 40µl. With your partner, research the science behind restriction enzymes. We also constructed the digestive system in our manikin. Your teacher will direct you to the heat block or water bath. Attach the rubric to your paper and submit. Use the micropipettor to dispense 10µl of Enzyme Reaction Buffer into each of the four labeled tubes.
Suggest reasons why it was helpful to digest each of your samples with two different restriction enzymes? It is also important to be able to follow specific procedure for dissecting the different structures in order to be able to view it properly once it is cut. Tap the tube gently on the desk to make sure all of the contents are in the bottom of the tube. Place your tubes in the bath as directed by your teacher. It is done by inserting and inflating a balloon in an artery at the site of a plaque by using a catheter. In Lesson 2, you used basic forensic anthropology to analyze bones and to provide a preliminary snapshot of the two individuals found in the park.
Abstract: In this activity, my group and I were selected to examine human remains of a parts found in a park. This is important in biomedical sciences because it is important to know how to help people who have blood clots or large plaques built up in their artery walls. The gel should take 30-45 minutes to run. This is done by measuring the increase in temperature of a known volume of water when a portion of the food is burned. While your samples cool, practice loading samples on the practice gels. Also, make sure to note the contents of each tube in your laboratory journal.
This is an important skill to have in the biomedical sciences because it is important to be an expert on the human body and be able to identify certain structures of different mammalian organs, including the heart. Remove the comb carefully from the agarose gel and place the gel in the electrophoresis chamber. Make sure to clearly indicate which sample is in which well. From this urinalysis, we had to diagnose the patients with the correct diagnosis. Finally, cut the heart almost in half, leaving a flap connecting the two halves. Unless you are an identical twin, there is no other person on the planet with your same code. Work carefully to identify these individuals and finally give their families some peace.
The student response sheet is found linked below. Draw a diagram of the gel in your lab notebook. We had to measure the angle of the pelvic bone and also the angle of the nose and eye circle. What is gel electrophoresis and how can the results of this technique be interpreted? If possible, attach a labeled photograph of your gel to the completed case report. It's also important to know that angioplasties are just a temporary fix also, and a stent insertion would be the next step. We completed a Case Study which is attached below.
We researched the different components of the digestive system and completed a student response sheet. We diagnosed Melissa with severe acute asthma. It is important to understand the flow of blood through a mammalian heart because it makes it easier to identify when and where problems may occur in blood flow. Depending on time, your teacher may complete this step for you. Incubate your tubes in a 37°C water bath for 45-60 minutes. Make sure to use a fresh tip for each sample.
Make sure the loading dye is migrating out of the well and moving towards the positive pole. As these fragments move, their varying lengths propel them through the gel at different speeds. After looking over her medical histories, we were asked to complete a diagnosis for her. Use the websites listed below to complete the questions and activities in Part A of the Student Response Sheet. We created a concept map to help describe this, which can be found below. We concluded our work by gathering all of the information in a client report, as posted to the right. During this time, continue work on the Student Resource Sheet.